Mouse Androgenetic Embryonic Stem Cells Differentiated to Multiple Cell Lineages in Three Embryonic Germ Layers In Vitro

作者：Teramura Takeshi^*; Onodera Yuta; Murakami Hideki; Ito Syunsuke; Mihara Toshihiro; Takehara Toshiyuki; Kato Hiromi; Mitani Tasuku; Anzai Masayuki; Matsumoto Kazuya; Saeki Kazuhiro; Fukuda Kanji; Sagawa Norimasa; Hosoi Yoshihiko

来源：Journal of Reproduction and Development, 2009, 55(3): 283-292.

DOI：10.1262/jrd.20146

• Androgenetic embryo
• Differentiation
• Embryonic stem cell

The embryos of some rodents and primates can precede early development without the process of fertilization; however, they cease to develop after implantation because of restricted expressions of imprinting genes. Asexually developed embryos are classified into parthenote/gynogenote and androgenote by their genomic origins. Embryonic stem cells (ESCs) derived from asexual origins have also been reported. To date, ESCs derived from parthenogenetic embryos (PgESCs) have been established in some species, including humans, and the possibility to be alternative sources for autologous cell transplantation in regenerative medicine has been proposed. However, some developmental characteristics, which might be important for therapeutic applications, such as multiple differentiation capacity and transplantability of the ESCs of androgenetic origin (AgESCs) are uncertain. Here, we induced differentiation of mouse AgESCs and observed derivation of neural cells, cardiomyocytes and hepatocytes in vitro. Following differentiated embryoid body (EB) transplantation in various mouse strains including the strain of origin, we found that the EBs Could engraft in theoretically MHC-matched strains. Our results indicate that AgESCs possess at least two important characteristics, multiple differentiation properties in vitro and transplantability after differentiation, and suggest that they can also serve as a source of histocompatible, tissues for transplantation.

• 出版日期2009-6