A semi-automated method for purification of milligram quantities of proteins on the QIAcube

作者：McGraw J; Tatipelli V K; Feyijinmi O; Traore M C; Eangoor P; Lane S; Stollar E J^*

来源：Protein Expression and Purification, 2014, 96: 48-53.

DOI：10.1016/j.pep.2014.01.014

• SH3 domain
• Protein purification
• High-throughput automation
• QIAcube
• Protein expression

A growing number of studies require the purification of multiple proteins simultaneously and the development of simple economical high-throughput purification methods is essential. We have tested the purification of two related proteins in a variety of conditions to benchmark the semi-automated affinity chromatography method for the QlAcube that we have developed. We find that this new QlAcube method can successfully purify milligram quantities of proteins with minimal user involvement and performs as well as methods based on gravity. The method could easily be adapted to other chromatography resins and should prove to be a versatile method for optimizing protein expression or purification conditions for multiple proteins while obtaining sufficient amounts for subsequent biochemical analyses.

• 出版日期2014-4