Ginsenosides Rb1 and Rg1 Stimulate Melanogenesis in Human Epidermal Melanocytes via PKA/CREB/MITF Signaling

作者：Lin, Mao; Zhang, Bao-Xiang; Zhang, Ci; Shen, Nan; Zhang, Yun-Ying; Wang, Ao-Xue; Tu, Cai-Xia^*

来源：Evidence-Based Complementary and Alternative Medicine, 2014, 2014: 892073.

DOI：10.1155/2014/892073

• B16 MELANOMA-CELLS
• MICROPHTHALMIA GENE-PRODUCT
• TRANSCRIPTION FACTOR
• NERVOUS-SYSTEM
• UP-REGULATION
• EXPRESSION
• TYROSINASE
• MITF
• DIFFERENTIATION
• ACTIVATION

Reduced or defective melanin skin pigmentation may cause many hypopigmentation disorders and increase the risk of damage to the skin triggered byUVirradiation. GinsenosidesRb1 andRg1 havemanymolecular targets including thecAMP-response elementbinding protein (CREB), which is involved in melanogenesis. This study aimed to investigate the effects of ginsenosides Rb1 and Rg1 on melanogenesis in human melanocytes and their related mechanisms. The effects of Rb1 and Rg1 on cell viability, tyrosinase activity, cellular melanin content and protein levels of tyrosinase, microphthalmia-associated transcription factor (MITF), and activation of CREB in melanocytes were assessed. Results showed that Rb1 or Rg1 significantly increased cellular melanin content and tyrosinase activity in a dose-dependent manner. By contrast, the cell viability of melanocytes remained unchanged. After exposure to Rb1 or Rg1, the protein levels of tyrosinase, MITF, and phosphorylated CREBwere significantly increased. Furthermore, pretreatment with the selective PKAinhibitor H-89 significantly blocked the Rb1-or Rg1-induced increase of melanin content. These findings indicated that Rb1 and Rg1 increased melanogenesis and tyrosinase activity in human melanocytes, which was associated with activation of PKA/CREB/MITF signaling. The effects and mechanisms of Rb1 or Rg1 on skin pigmentation deserve further study.

• 出版日期2014
• 单位大连医科大学