Deciphering cellular functions of protein phosphatases by comparison of gene expression profiles in Saccharomyces cerevisiae

作者：Hirasaki Masataka; Nakamura Fumika; Yamagishi Kazuo; Numamoto Minori; Shimada Yukiko; Uehashi Keigo; Muta Shigeru; Sugiyama Minetaka; Kaneko Yoshinobu; Kuhara Satoru; Harashima Satoshi^*

来源：Journal of Bioscience and Bioengineering, 2010, 109(5): 433-441.

DOI：10.1016/j.jbiosc.2009.10.023

• Saccharomyces cerevisiae: Protein phosphatase
• Expression profile
• Pearson's correlation
• Phenotypic analysis
• Hygromycin B
• Congo red
• Calcofluor white

Expression profiles of protein phosphatase (PPase) disruptants were analyzed by use of Pearson's correlation coefficient to find profiles that correlated with those of 316 Reference Gene (RG) disruptants harboring deletions in genes with known functions. Twenty-six Appose disruptants exhibited either a positive or negative correlation with 94 RG disruptants when the p value for Pearson's correlation coefficient was >0.2. Some of the predictions that arose from this analysis were tested experimentally and several new Apprise phenotypes were found. Notably, Delta sit4 and Delta siw14 disruptants exhibited hygromycin B sensitivity, Delta sit4 and Delta ptc1 disruptants grew slowly on glycerol medium, the Delta ptc1 disruptant was found to be sensitive to calcofluor white and congo red, while the Delta ppg1 disruptant was found to be sensitive to congo red. Because on-going analysis of expression profiles of Saccharomyces cerevisiae disruptants is rapidly generating new data, we suggest that the approach used in the present study to explore PPase function is also applicable to other genes.

• 出版日期2010-5