摘要

To evaluate if UV-LEDs can be a good candidate for sterilization of bacteria, Escherichia colt, Salmonella typhimurium, Staphylococcus aureus, Bacillus cereus and Vibrio parahaemolyticus were cultivated in a Luria-Bertani (LB) broth and UV-LEDs were directly irradiated on LB broth with bacteria or on LB agar plate where the diluted LB broth with bacteria was spread. Three different wavelengths of UV LEDs, 365, 310 and 265 nin defined as the UV-A, UV-B and UV-C region, respectively were used for the irradiation. The data obtained showed that E. coli in the LB broth did not increase with the passage of time under irradiation with 265 and 310 nm UV light while they were significantly increased under irradiation of 365 nm UV light. On the other hand, E. coli on the LB agar plate decreased with the passage of time, having a half-life of similar to 2 hr under 365 nm, similar to 15 min under 310 nm and similar to 10 sec under 265 nm irradiation, respectively. Salmonella typhimurium, S. aureus, B. cereus and V parahaemolyticus onto the LB agar plates decreased with the passage of time only under 265 nm irradiation, having a half-life of similar to 15 min, similar to 1.5 hr, similar to 45 min and similar to 5 min, respectively. These results suggested that the UV-LEDs can be a good candidate for sterilization of bacteria.

  • 出版日期2014-6