Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates

作者：Botcherby Edward J; Smith Christopher W; Kohl Michael M; Debarre Delphine; Booth Martin J; Juskaitis Rimas; Paulsen Ole; Wilson Tony^*

来源：Proceedings of the National Academy of Sciences, 2012, 109(8): 2919-2924.

DOI：10.1073/pnas.1111662109

• fluorescence microscopy
• multiphoton imaging
• multiphoton microscopy
• three-dimensional microscopy

Multiphoton microscopy is a powerful tool in neuroscience, promising to deliver important data on the spatiotemporal activity within individual neurons as well as in networks of neurons. A major limitation of current technologies is the relatively slow scan rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate how this high-speed 3D multiphoton imaging system can be used to study neuronal activity at millisecond resolution at the subcellular as well as the population level.

• 出版日期2012-2-21