Toll-Like Receptor 4 Has an Essential Role in Early Skin Wound Healing

作者：Chen Lin; Guo Shujuan; Ranzer Matthew J; DiPietro Luisa A^*

来源：Journal of Investigative Dermatology, 2013, 133(1): 258-267.

DOI：10.1038/jid.2012.267

Toll-like receptor 4 (TLR4) has a key role in the initiation of innate immunity and in the regulation of adaptive immune responses. Using microarray analysis and PCR, TLR4 expression was observed to increase in murine skin wounds at the early stages. The cellular location of TLR4 was primarily in keratinocytes at the wound edges. The closure of excisional wounds was significantly delayed in TLR4-deficient (C3H/HeJ) as compared with wild-type mice, and both IL-1 beta and IL-6 production were significantly lower in the wounds of TLR4-deficient mice. EGF also markedly decreased in the wound edge of epidermis in TLR4-deficient mice. In vitro studies confirmed that a wound stimulus induces TLR4 mRNA expression in primary normal human epidermal keratinocytes (NHEK). In vitro injury also induced the phosphorylation of p38 and JNK MAPK (Jun N-terminal kinase mitogen-activated protein kinase) and the expression of IL-1 beta and tumor necrosis factor-alpha by NHEK. Blockade of TLR4 delayed NHEK migration and abolished the phosphorylation of p38 and INK MAPK, and blockade of TLR4 and/or p38/JNK abolished IL-1 beta production. The results suggest that inflammatory cytokine production by injured NHEK is stimulated via the TLR4-p38 and JNK MARK signaling pathway. Together, the results provide evidence for a role of TLR4 at sites of injury, and suggest that TLR4 is an important regulator of wound inflammation. Journal of Investigative Dermatology (2013) 133, 258-267; doi:10.1038/jid.2012.267; published online 6 September 2012

• 出版日期2013-1