Actin Depletion Initiates Events Leading to Granule Secretion at the Immunological Synapse

作者：Ritter Alex T; Asano Yukako; Stinchcombe Jane C; Dieckmann N M G; Chen Bi Chang; Gawden Bone C; van Engelenburg Schuyler; Legant Wesley; Gao Liang; Davidson Michael W; Betzig Eric; Lippincott Schwartz Jennifer^*; Griffiths Gillian M

来源：Immunity, 2015, 42(5): 864-876.

DOI：10.1016/j.immuni.2015.04.013

Cytotoxic T lymphocytes (CTLs) use polarized secretion to rapidly destroy virally infected and tumor cells. To understand the temporal relationships between key events leading to secretion, we used high-resolution 4D imaging. CTLs approached targets with actin-rich projections at the leading edge, creating an initially actin-enriched contact with rearward-flowing actin. Within 1 min, cortical actin reduced across the synapse, T cell receptors (TCRs) clustered centrally to form the central supramolecular activation cluster (cSMAC), and centrosome polarization began. Granules clustered around the moving centrosome within 2.5 min and reached the synapse after 6 min. TCR-bearing intracellular vesicles were delivered to the cSMAC as the centrosome docked. We found that the centrosome and granules were delivered to an area of membrane with reduced cortical actin density and phospholipid PIP2. These data resolve the temporal order of events during synapse maturation in 4D and reveal a critical role for actin depletion in regulating secretion.

• 出版日期2015-5-19
• 单位河北医科大学