Determination of (3)J((1)H3 '-P-31) couplings in a DNA oligomer with enhanced sensitivity employing a constant-time TOCSY difference experiment

作者：Reith Lorenz M; Schlagnitweit Judith; Smrecki Vilko; Knoer Guenther; Mueller Norbert; Schoefberger Wolfgang^*

来源：Magnetic Resonance in Chemistry, 2011, 49(3): 125-128.

DOI：10.1002/mrc.2729

• NMR
• constant time TOCSY experiment
• DNA oligomer
• (3)J((1)H3 '-P-31) coupling constants

A constant-time TOCSY difference experiment for the determination of (3)J((1)H3'-P-31) coupling constants in non-isotope-labelled DNA oligonucleotides is presented. The method is tested on a DNA octamer and compared with the established constant-time NOESY difference method. Each (3)J((1)H3'-P-31) coupling constant is determined from amplitude changes caused by phosphorous decoupling, which are observable on multiple cross-peaks, thus leading to a high accuracy of the value of the (3)J((1)H3'-P-31) coupling constant. The new experiment delivers up to three times the sensitivity compared with previously reported methods.

• 出版日期2011-3