Activation of PPAR alpha and PPAR beta/delta regulates Sertoli cell metabolism

作者：Regueira M; Riera M F; Galardo M N; Pellizzari E H; Cigorraga S B; Meroni S B^*

来源：Molecular and Cellular Endocrinology, 2014, 382(1): 271-281.

DOI：10.1016/j.mce.2013.10.006

• Fatty acid oxidation
• Pyruvate Dehydrogenase Complex
• Lactate
• Testis

The purpose of this study was to evaluate the existence of a possible simultaneous regulation of fatty acid (FA) metabolism and lactate production by PPAR a and PPAR beta/delta activation in Sertoli cells (SC). SC cultures obtained from 20-day-old rats were incubated with WY14643 or GW0742 pharmacological activators of PPAR alpha and PPAR beta/delta respectively. The fatty acid transporter CD36, carnitine palmitoyltransferase 1, long- and medium-chain 3-hydroxyacyl-00A dehydrogenases mRNA levels were analyzed. An increase in the above-mentioned genes in response to activation of both nuclear receptors was observed. Additionally, PPAR beta/delta activation increased lactate production as a consequence of increased pyruvate availability by inhibiting the Pyruvate Dehydrogenase Complex. Altogether, these results suggest that in SC, PPAR alpha activation participates in the regulation of FA metabolism. On the other hand, PPAR beta/delta activation regulates FA metabolism and lactate production ensuring simultaneously the energetic metabolism for SC and germ cells.

• 出版日期2014-1-25