Development of validated high-temperature reverse-phase UHPLC-PDA analytical method for simultaneous analysis of five natural isothiocyanates in cruciferous vegetables

作者：Robin; Arora Rohit; Arora Saroj; Vig Adarsh Pal^*

来源：Food Chemistry, 2018, 239: 1085-1089.

DOI：10.1016/j.foodchem.2017.07.059

• UHPLC
• Isothiocyanates
• Temperature
• Brassica rapa var. rapa L.
• Raphanus sativus L. var. oleiformis Pers.
• Eruca sativa Mill

In the present study reverse-phase UHPLC-PDA technique was developed at 60 degrees C for simultaneous quantification of allyl, 3-butenyl, 4-(methylthio)butyl, benzyl and phenethyl isothiocyanates. The validation parameter showed a very good linearity, with a correlation coefficient of 1.00 for all detected standard analytes. Also, high precision and accuracy were observed with lowest obtained values of 1.39% and 99.1%, respectively. Different varieties of three plants, viz. Brassica rapa var. rapa L., Raphanus sativus L. var. oleiformis Pers. and Eruca sativa Mill., were analyzed with this method. After analysis, 4-(methylthio) butyl isothiocyanate was observed to be the major component in the varieties of arugula. Allyl, benzyl and phenethyl isothiocyanates were detected in turnip varieties and, in addition, 3-butenyl isothiocyanate was detected in radish varieties.

• 出版日期2018-1-12