GROWTH AND COMPOSITION OF Arthrospira (Spirulina) platensis IN A TUBULAR PHOTOBIOREACTOR USING AMMONIUM NITRATE AS THE NITROGEN SOURCE IN A FED-BATCH PROCESS

作者：Cruz Martinez L C; Jesus C K C; Matsudo M C; Danesi E D G; Sato S; Carvalho J C M^*

来源：Brazilian Journal of Chemical Engineering, 2015, 32(2): 347-356.

DOI：10.1590/0104-6632.20150322s00003062

• Arthrospira (Spirulina) platensis
• Fed-batch cultivation
• Ammonium nitrate
• Light intensity
• Tubular photobioreactor

NH4NO3 simultaneously provides a readily assimilable nitrogen source (ammonia) and a reserve of nitrogen (nitrate), allowing for an increase in Arthrospira platensis biomass production while reducing the cost of the cultivation medium. In this study, a 2(2) plus star central composite experimental design combined with response surface methodology was employed to analyze the influence of light intensity (I) and the total amount of added NH4NO3 (M-t) on a bench-scale tubular photobioreactor for fed-batch cultures. The maximum cell concentration (X-m), cell productivity (P-X) and biomass yield on nitrogen (Y-X/N) were evaluated, as were the protein and lipid contents. Under optimized conditions (I = 148 mu mol.photons.m(-2).s(-1) and M-t = 9.7 mM NH4NO3), X-m = 4710 +/- 34.4 mg.L-1, P-X = 478.9 +/- 3.8 mg.L-1.d(-1) and Y-X/N = 15.87 +/- 0.13 mg.mg(-1) were obtained. The best conditions for protein content in the biomass (63.2%) were not the same as those that maximized cell growth (I = 180 mu mol.photons.m(-2).s(-1) and M-t = 22.5 mM NH4NO3). Based on these results, it is possible to conclude that ammonium nitrate is an interesting alternate nitrogen source for the cultivation of A. platensis in a fed-batch process and could be used for other photosynthetic microorganisms.

• 出版日期2015-6