Arabidopsis receptor-like cytoplasmic kinase BIK1: purification, crystallization and X-ray diffraction analysis

作者：Lal Neeraj K; Fisher Andrew J^*; Dinesh Kumar Savithramma P^*

来源：Acta Crystallographica Section F-Structural Biology Communications, 2016, 72(10): 738-742.

DOI：10.1107/S2053230X16013522

• receptor-like kinase
• receptor-like cytoplasmic kinases
• RLCK
• BIK1
• BOTRYTIS-INDUCED KINASE 1
• PAMP-triggered immunity
• Arabidopsis

Receptor-like cytoplasmic kinases (RLCKs) in Arabidopsis play a central role in the integration of signaling input from various growth and immune signaling pathways. BOTRYTIS-INDUCED KINASE 1 (BIK1), belonging to the RLCK family, is an important player in defense against bacterial and fungal pathogens and in ethylene and brassinosteroid hormone signaling. In this study, the purification and crystallization of a first member of the class VI family of RLCK proteins, BIK1, are reported. BIK1 was crystallized using the microbatch-under-oil method. X-ray diffraction data were collected to 2.35 angstrom resolution. The crystals belonged to the monoclinic space group C2, with two monomers per asymmetric unit.

• 出版日期2016-10