科研之友
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摘要
Brucella sp. are pathogenic bacteria that are internalized by host cells upon activation of cell division cycle 42 (Cdc42) to cause Brucellosis. Buffalo are among the many species that are affected by Brucellosis. The objective of this study was to analyze the expression and distribution of Cdc42 small GTPases in buffalo which may help elucidating the molecular events leading to Brucella internalization Full-length buffalo Cdc42 mRNA were obtained and real-time quantitative polymerase chain reaction was performed to assess the transcription level of Cdc42 in heart, liver, spleen, lung, kidney and intestine. Western blot was used to determine the level of Cdc42 in heart, liver, spleen, lung, kidney and intestine tissue; immunohistochemistry techniques were applied for evaluating the distribution of Cdc42 in tissue. Cdc42 were found to be expressed in heart, liver, spleen, lung, kidney and intestine tissue and the subcellular localization of Cdc42 was predominantly in the cytoplasma. These data provide important anatomical information on the role of Cdc42 in Brucella internalization in the different buffalo tissue.
