科研之友
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摘要
A sensitive assay was demonstrated for the colorimetric and luminescent detection of heparin. Positively charged [Ru(phen) 2np](2+) (np: naphtho[2,3- f][1,10] phenanthroline) can interact with negatively charged AuNPs through electrostatic interaction, leading to the luminescence quenching of [Ru(phen) 2np](2+) via energy transfer as well as the aggregation of AuNPs together with a color change in solution from red to blue. Upon the addition of negatively charged heparin to gold nanoparticles functionalized by ruthenium(II) complexes, the emission of [Ru(phen) 2np](2+) recovered due to the competitive adsorption of [Ru(phen) 2np](2+) to heparin and AuNPs. A linear calibration curve was obtained in the 0.4 mu M to 100 mu M range and 0.1 mu M to 6 mu M with a low detection limit of 0.22 mu M and 0.024 mu M based on 3s/s for heparin quantification in buffer solution and in 1% fetal bovine serum (FBS), respectively. Interestingly, the naked eye alone can judge the presence of 0.02 mu M heparin without the aid of any advanced instruments by color change. Thus a convenient and visual method to detect heparin was established.
- 出版日期2017-10-17
- 单位福州大学
