A novel pyrimidine tetrad contributing to stabilize tetramolecular G-quadruplex structures

作者：Esposito V; Pepe A; Filosa R; Mayol L; Virgilio A^*; Galeone A^*

来源：Organic and Biomolecular Chemistry, 2016, 14(10): 2938-2943.

DOI：10.1039/c5ob02358k

G-quadruplex structures formed by oligodeoxyribonucleotides TGGU(NH2)GGT (AM, U-NH2 = 5-amino-2'-deoxyuridine), TGGU(Br)GGT (BR, U-Br = 5-bromo-2'-deoxyuridine) and TGGTGGT (TH) have been investigated through circular dichroism, nuclear magnetic resonance, gel electrophoresis and molecular modeling techniques. Collected data indicate that all 7-mer oligonucleotides form tetramolecular parallel G-quadruplex structures with all residues adopting anti glycosidic bonds. In the case of AM, data suggest the occurrence of a novel U-NH2-tetrad characterized by eight hydrogen bonds that stabilizes the G-quadruplex structure more efficiently than U-Br-and T-tetrads.

• 出版日期2016