A Simple and Fast Method for the Determination of 20 Veterinary Drug Residues in Bovine Kidney and Liver by Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry

作者：Rizzetti Tiele M; de Souza Maiara P; Prestes Osmar D; Adaime Martha B; Zanella Renato

来源：Food Analytical Methods, 2017, 10(4): 854-864.

DOI：10.1007/s12161-016-0649-5

• Multi-class
• Veterinary drugs
• Kidney
• Liver
• UHPLC-MS/MS

A simple and rapid sample preparation method was developed and validated for multi-class analysis of veterinary drug residues in bovine kidney and liver. Sample preparation procedure was performed using acetonitrile and trichloroacetic acid for protein precipitation followed by ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) analysis. The proposed method was validated according to the criteria of the Commission Decision 2002/657/EC evaluating linearity, selectivity, accuracy and precision, determination limit (CC alpha), and detection capacity (CC beta). Linearity presented r (2) ae 0.99 for all the target compounds, and recoveries ranged from 80 to 110 % with RSD ae17 % for intra- and inter-day assay. Values of CC alpha and CC beta ranged from 11 to 1096 and from 12 to 1191 mu g kg(-1), respectively. The proposed sample preparation followed by UHPLC-MS/MS analysis was suitable for the determination of 20 veterinary drug residues in bovine kidney and liver in routine analysis. Method applicability was evaluated using commercial samples.

• 出版日期2017-4