A New Approach of Rpf Addition to Explore Bacterial Consortium for Enhanced Phenol Degradation Under High Salinity Conditions

作者：Li, Ziqiao; Zhang, Yunge; Wang, Yuyang; Mei, Rongwu; Zhang, Yu; Hashmi, Muhammad Zaffar; Lin, Hongjun; Su, Xiaomei^*

来源：Current Microbiology, 2018, 75(8): 1046-1054.

DOI：10.1007/s00284-018-1489-x

• RESUSCITATION-PROMOTING FACTOR
• EXTRACELLULAR ORGANIC-MATTER
• NON-CULTURABLE BACTERIA
• MICROCOCCUS-LUTEUS
• WASTE-WATER
• MICROBIAL COMMUNITY
• AROMATIC-COMPOUNDS
• BIODEGRADATION
• BIPHENYL
• REMOVAL

Only a small fraction of salt-tolerant phenol-degrading bacteria can be isolated by conventional plate separation methods, because most bacteria in nature are in a viable but non-culturable (VBNC) state. The aims of this study were to screen out more effective functional bacteria using resuscitation-promoting factor (Rpf), and to determine whether a mixed bacterial consortium possesses better phenol-degrading capabilities under high salinity conditions. The results indicated that three strains unique to treatment group with Rpf addition were obtained. A mixed bacterial consortium consisting of two high-efficient strains which belonged to genera Bacillus and Corynebacterium was capable of utilizing phenol as a sole source of carbon at high salinity. Complete degradation of 100 mg/L phenol at 2% NaCl concentration was achieved within 8 h. This study provides new insights into resuscitation of VBNC bacteria for enhanced treatment of phenol-laden saline wastewater.

• 出版日期2018-8
• 单位浙江师范大学; 浙江省环境保护科学设计研究院