A new method of duplication of the chronic intraocular hypertension model: underwater electrocoagulation

作者：Lue Hong Bin; Li Yan; Yuan Yuan Sheng; Luo Qing Li

来源：International Journal of Ophthalmology, 2008, 1(1): 45-47.

• glaucoma
• intraocular hypertension
• animal model

AIM: To study the experimental method of inducing the chronic intraocular hypertension in rat eyes.
METHODS: Twenty Wistar rats were randomly divided into ocular hypertension and sham control groups. Intraocular pressure (IOP) was raised by electrocoagulating at least 3 episcleral and limbal veins on the right eye of each rat in ocular hypertension group and its contralateral eye was used as control. At 1, 2, 3, 4 and 8 week after the electrocoagulation of the veins, TOP were measured.
RESULTS: The treatment of electrocoagulation caused a significant TOP increase of the right eyes over the baseline, over the contralateral eyes, and over the sham control eyes (repeated measures ANOVA, P<0.001). At 1 week, IOP was 30.12+/-5.18mmHg, and maintained the high TOP up to 8 weeks.
CONCLUSION: The chronic intraocular hypertension model could be successfully created by electrocoagulating three or more episcleral and limbal veins.

• 出版日期2008-3-18
• 单位昆明医科大学; 四川大学