An evidence in vitro for the influence of bisphenol A on uterine leiomyoma

作者：Shen, Yang^*; Ren, Mu-Lan; Feng, Xu; Cai, Yun-Lang; Gao, Yong-Xing; Xu, Qian

来源：European Journal of Obstetrics & Gynecology and Reproductive Biology, 2014, 178: 80-83.

DOI：10.1016/j.ejogrb.2014.03.052

• Uterine leiomyoma
• Environmental estrogen
• Bisphenol A
• ER alpha
• IGF-1
• VEGF

Objective: To study the effect and mechanism of environmental estrogens bisphenol A (BPA) on uterine leiomyoma (UL) cells. @@@ Methods: Primary cultures and subcultures of human UL cells, which were identified by immunocytochemical staining with a monoclonal anti-a-smooth muscle actin antibody, were performed. The viability of cells in different concentration of bisphenol A of 24 h, 48 hand 72 h were analyzed by CCK-8. The expressions of mRNA of ER alpha, IGF-1 and VEGF in all groups were detected by real-time quantitative PCR assay, and then the expressions of proteins detected by Western blot assay. @@@ Results: BPA promoted the growth of UL cells and the expressions of ER alpha, IGF-1 and VEGF, which had positive correlation with the concentration and action time of BPA treatment. @@@ Conclusion: BPA promotes the growth of leiomyoma cells. The expressions of IGF-1, VEGF can be up-regulated by ER alpha, which may be possible mechanism of BPA promote the growth of leiomyoma cells.

• 出版日期2014-7
• 单位东南大学