A low-cost miniaturized potentiostat for point-of-care diagnosis

作者：Cruz Andres Felipe Diaz; Norena Nicolas; Kaushik Ajeet; Bhansali Shekhar^*

来源：Biosensors and Bioelectronics, 2014, 62: 249-254.

DOI：10.1016/j.bios.2014.06.053

• Miniaturized potentiostat
• Point-of-care
• Personalized health monitoring/diagnostic
• Electrochemical immunosensing
• Cortisol

This paper presents a novel approach of using a miniaturized potentiostat (M-P) chip (LMP91000) to perform full range cyclic voltammetry (CV) measurements for the detection of biomarkers. The LMP91000 evaluation board was reconfigured to perform three-electrode CV measurements in order to achieve electrochemical cortisol immunosensing. The microelectrodes for cortisol estimation were fabricated by immobilizing monoclonal anti-cortisol antibody (Anti-M-Cab) onto self-assembled monolayer (SAM) modified Au microelectrodes. The results obtained using the M-P were compared to those obtained using a conventional potentiostat. The M-P was successful in measuring cortisol levels in the range of pM. The outcomes of the studies suggest that M-P can effectively perform biochemical measurements on three electrode systems, enabling the development of miniature systems for point-of-care (POC) diagnosis.

• 出版日期2014-12-15