Genetic relationships of Bacillus anthracis and closely related species based on variable-number tandem repeat analysis and BOX-PCR genomic fingerprinting

作者：Kim W; Hong YP; Yoo JH; Lee WB; Choi CS; Chung SI^*

来源：FEMS Microbiology Letters, 2002, 207(1): 21-27.

DOI：10.1111/j.1574-6968.2002.tb11022.x

• variable-number tandem repeats
• BOX-PCR genomic fingerprinting
• Bacillus anthracis
• Bacillus cereus group

Variable-number tandem repeats (VNTR) analysis and BOX-repeat-based PCR (BOX-PCR) genomic finger-printing were performed on 25 Bacillus strains to investigate the genetic relatedness of Bacillus anthracis to the closely related species. Based on VNTR analysis, all B, anthracis strains could be assigned to (VNTR)(4), which is the most commonly found type in the world. Interestingly, a (VNTR)(2) was also observed in Bacillus cereus KCTC 1661 and with an exact match to the tandem repeats found in B. anthracis. This finding has never been reported before in the closely related species. According to the BOX-PCR, B. anthracis strains clustered together and separated reliably from the closely related species. However, B. cereus KCTC 1661 was linked to the B. anthracis cluster and showed close relationships with B. anthracis strains. These results indicated that there was a strong correlation between VNTR analysis and BOX-PCR genomic fingerprinting.

• 出版日期2002-1-22
• 单位河北医科大学