NEW MICROSATELLITE LOCI FOR POMEGRANATE, PUNICA GRANATUM (LYTHRACEAE)

作者:Curro Sergio; Caruso Marco; Distefano Gaetano; Gentile Alessandra; La Malfa Stefano*
来源:American Journal of Botany, 2010, 97(7): E58-E60.
DOI:10.3732/ajb.1000143

摘要

Premise of the study: A new set of pomegranate microsatellites was selected and characterized to assess the level of genetic diversity among cultivars and wild genotypes. Methods and Results: Nine Simple Sequence Repeat (SSR) markers were obtained using the Microsatellite-AFLP technique and were successfully amplified in 34 genotypes belonging to Italian, Spanish, and Turkish germplasm collections. The number of alleles per locus ranged from 1 to 5, and the total number of alleles was 22. Conclusions: Because only a few codominant markers are available for this species, the newly identified SSRs will facilitate genetic diversity studies, fingerprinting, and mapping. In addition, the 9 loci successfully amplified in P. granatum var. nana. No cross transferability was observed for Cuphea micropetala and Lagerstroemia indica (Lythraceae).

  • 出版日期2010-7