Molecular characterization of a SCAR marker purportedly linked to seedlessness in grapevine (Vitis)

作者：Li Zhijian T; Dhekney S A; Gray D J^*

来源：Molecular Breeding, 2010, 25(4): 637-644.

DOI：10.1007/s11032-009-9361-0

• SCAR marker
• Seedlessness
• Genomic sequence
• PCR
• Histidine triad protein gene
• Vitis vinifera

Molecular markers associated with seedlessness in grapevine (Vitis vinifera) could be used for early trait selection in breeding programs and provide useful tools for elucidating the molecular mechanisms underlying seed abortion. In this study, the sequence fidelity of a previously reported seedlessness-linked Sequence-Characterized Amplified Region (SCAR) marker (GenBank accession #AY327513, Yang et al. in Chinese J Agri Biotech 3:13-17, 2006) was determined by comparative sequence analysis. Using available genomics resources, the SCAR marker was mapped to an overlapping region between exon-1 and intron-1 of a putative histidine triad protein (HIT) gene corresponding to an ORF of 588 bp for a peptide of 195 residues and a gene structure with 8 exons in a 14.7 kbp genomic region. However, PCR and DNA sequence analyses revealed that both seeded and seedless phenotypes of Vitis species contained highly homologous sequences in an EcoRI-delimited region of up to 5 kbp surrounding the SCAR marker. The absence of linkage between the marker and the seedlessness trait does not support a role for the HIT gene in seedlessness development, or the potential for the use of this SCAR marker in the identification/selection of seedless progeny.

• 出版日期2010-4