ALDH1-positive cancer stem-like cells are enriched in nodal metastases of oropharyngeal squamous cell carcinoma independent of HPV status

作者：Qian Xu; Wagner Steffen; Ma Chenming; Klussmann Jens P; Hummel Michael; Kaufmann Andreas M; Albers Andreas E^*

来源：Oncology Reports, 2013, 29(5): 1777-1784.

DOI：10.3892/or.2013.2340

• squamous cell carcinoma
• human papillomavirus
• cancer stem cell
• aldehyde-dehydrogenase
• metastasis

Oropharyngeal squamous cell carcinoma (OSCC) is caused by high-risk (HR) human papillomavirus (HPV) or alcohol and tobacco abuse. Aldehyde dehydrogenase 1 (ALDH1) is a confirmed marker for cancer stem-like cells (CSCs) of OSCC responsible for therapy resistance, recurrence and metastasis. Associations between HR-HPV/p16, CSC frequency and clinicopathological parameters in patients with metastatic OSCC were investigated. In the present study, HPV genotypes and expression of ALDH1 and p16 was analyzed in 40 paired OSCC and metastases. A significant correlation between ALDH1 positivity with lower primary tumor differentiation grade (P=0.009) and higher nodal status (P=0.015) was noted. Compared to primary tumors, the proportion of ALDH1-expressing cells was significantly increased in metastases (P=0.012), while significantly fewer ALDH1-expressing cells were found in HR-HPV-DNA(+)/p16(+) primary tumors (P=0.038) compared to HR-HPV-DNA(-)/p16(-) primary tumors. Metastases showed no difference. ALDH1(+) CSCs are detectable in OSCC and metastases. ALDH1 high-grade OSCC exhibits a more aggressive phenotype characterized by higher nodal classification and lower differentiation. This suggests a subpopulation contained in the ALDH1-positive OSCC cell pool able to complete the metastatic cascade and subsequently enriching in metastasis independent of tumor etiology and ALDH1 content.

• 出版日期2013-5
• 单位温州医科大学