A novel glycosaminoglycan-like polysaccharide from abalone Haliotis discus hannai Ino: Purification, structure identification and anticoagulant activity

作者：Li, Guoyun; Chen, Shiguo; Wang, Yuming; Xue, Yong; Chang, Yaoguang; Li, Zhaojie; Wang, Jingfeng; Xue, Changhu^*

来源：International Journal of Biological Macromolecules, 2011, 49(5): 1160-1166.

DOI：10.1016/j.ijbiomac.2011.09.017

• Abalone
• Glycosaminoglycan
• Purification
• NMR analysis
• Anticoagulant

A novel glycosaminoglycan-like sulfated polysaccharide (AAP) from the pleopods of Haliotis discus hannai Ino was purified by DEAE ion exchange chromatography followed with S-300 HR geltrion chromatography. Chemical composition analysis indicated that MP was composed of galactosamine, glucuronic acid, fucose, galactose with a ratio of 2.14:2.37:2.94:1, the content of sulfate was 15.5%. The average molecular weight (M-w) was 56.2 kDa in a TSK G4000 column. Further IR, 1D, 2D NMR spectroscopic analysis of the correlation signals of different sugar units gave a proposal repeating structure, as follows:
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In vitro anticoagulant assay indicated AAP prolonged both the activated partial thromboplastin time (APT) and thrombin time (TT), with a 22.5 U/mg and 72.0 U/mg compared with standard heparin, respectively. The anticoagulant property of AAP was mainly attributed to powerful potentiation thrombin by anti-thrombin III.

• 出版日期2011-12-1
• 单位中国海洋大学