科研之友
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摘要
The interspecific genetic introgression approach has been shown to facilitate the detection and dissection of quantitative trait loci (QTL). A population consisting of 121 F(6) recombinant inbred lines was developed by crossing Gossypium hirsutum cv. 'Handan 208' and G. barbadense cv. 'Pima 90' via single-seed descent. Genotyping indicated that the mean 'Pima 90' allele frequency at each locus was 21%. Phenotyping and phenotypic distribution indicated a trend of return of individual lines' characters to 'Handan 208' coupled with a wide variance for each trait. Significant loci influencing fibre quality were detected by one-way analysis of variance (ANOVA; P < 0.005) and association analysis [-log(10)(P) >= 3]: five and three markers for fibre length, four and one marker(s) for uniformity, two and one marker(s) for micronaire, 13 and 15 markers for strength, six and 10 markers for elongation, respectively. Two-way ANOVA based on genotypes of all marker loci combination showed that 807 two-locus combinations were significant, and two-way ANOVA based on marker genotypes of QTL markers combination showed five significant digenic interactions (P < 0.01).
