Differential Proteomic Analysis of Renal Cell Carcinoma Tissue Interstitial Fluid

作者：Teng Pang ning; Hood Brian L; Sun Mai; Dhir Rajiv; Conrads Thomas P^*

来源：Journal of Proteome Research, 2011, 10(3): 1333-1342.

DOI：10.1021/pr101074p

• renal cell carcinoma
• tissue interstitial fluid
• proteomics
• biomarker
• cancer

Renal cell carcinoma (RCC), the most common type of kidney cancer, currently has no biomarker of clinical utility. The present study utilized a mass spectrometry-based proteomics workflow for identifying differentially abundant proteins in RCC by harvesting shed and secreted proteins from the tumor micro-environment through sampling tissue interstitial fluid (TIF) from radical nephrectomies. Matched tumor and adjacent normal kidney (ANK) tissues were collected from 10 patients diagnosed with dear cell RCC. One-hundred thirty-eight proteins were identified with statistically significant differential abundances derived by spectral counting in tumor TIP when compared to ANK TIF. Among those proteins with elevated abundance in tumor TT, nicotinamide n-methyltransferase (NNMT) and enolase 2 (ENO2) were verified by Western blot and selected reaction monitoring (,SRM). The presence of ENO2 and thrombospondin-1 (TSP1) were verified as present and at elevated abundance in RCC patient serum samples as compared to a pooled standard control by enzyme-linked immunosorbent assay (ELISA), recapitulating the relative abundance increase in RCC as compared with ANK TIP.

• 出版日期2011-3