A Limonoid Kihadanin B from Immature Citrus unshiu Peels Suppresses Adipogenesis through Repression of the Akt-FOXO1-PPAR gamma Axis in Adipocytes

作者：Baba Shizuka; Ueno Yasuaki; Kikuchi Takashi; Tanaka Reiko; Fujimori Ko

来源：Journal of Agricultural and Food Chemistry, 2016, 64(51): 9607-9615.

DOI：10.1021/acs.jafc.6b04521

• adipocyte
• Akt
• Citrus
• FOXO1
• limonoid

Citrus limonoids are secondary metabolites and exhibit a variety of biological activities. In this study, we elucidated the suppression of adipogenesis by a Citrus limonoid kihadanin B and determined its molecular mechanism in mouse 3T3-L1 adipocytes. Kihadanin B was purified from the peels of immature Citrus unshiu by HPLC, and its chemical structure was determined by NMR and mass spectrometry. Kihadanin B reduced the lipid accumulation with the reduction of the expression levels of the adipogenic and lipogenic genes, but did not affect lipolysis in adipocytes. Phosphorylation levels of Akt and a forkhead transcriptional factor, FOXO1, a repressor of PPAR gamma, were lowered by kihadanin B. Furthermore, kihadanin B increased the binding level of FOXO1 to the PPAR gamma gene promoter in adipocytes. These results indicate that a Citrus limonoid kihadanin B repressed the adipogenesis by decreasing lipid accumulation through the suppression of the Akt-FOXO1-PPAR gamma axis in 3T3-L1 adipocytes.

• 出版日期2016-12-28