N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier

作者：Guan Ziqiang; Delago Antonia; Nus**aum Phillip; Meyer Benjamin; Albers Sonja Verena; Eichler Jerry^*

来源：FEBS LETTERS, 2016, 590(18): 3168-3178.

DOI：10.1002/1873-3468.12341

• archaea
• dolichol
• N-glycosylation
• Sulfolobus acidocaldarius
• thermoacidophile

N-glycosylation is a post-translational modification that occurs across evolution. In the thermoacidophilic archaea Sulfolobus acidocaldarius, glycoproteins are modified by an N-linked tribranched hexasaccharide reminiscent of the N-glycans assembled in Eukarya. Previously, hexose-bearing dolichol phosphate was detected in a S. acidocaldarius Bligh-Dyer lipid extract. Here, we used a specialized protocol for extracting lipid-linked oligosaccharides to detect a dolichol pyrophosphate bearing the intact hexasaccharide, as well as its biosynthetic intermediates. Furthermore, evidence for N-glycosylation of two S. acidocaldarius proteins by the same hexasaccharide and its derivatives was collected. These findings thus provide novel insight into archaeal N-glycosylation.

• 出版日期2016-9