Antibodies against Shigella flexneri adhesion molecule outer membrane protein (OMP) can cross-react with OMPs of some Shigella species

作者：Milliana Alvi; Noorhamdani A S; Poeranto; Handono Kusworini; Sumarno; Prawiro Reto^*; Fitrianingsih Avin Ainur; Rachma Lailia Nur

来源：Tropical Journal of Pharmaceutical Research, 2017, 16(2): 255-262.

DOI：10.4314/tjpr.v16i2.1

• Shigella flexneri OMP
• Outer membrane protein
• Protein adhesion
• Antibody
• Cross-reaction

Purpose: To identify a Shigella flexneri hemagglutinin outer membrane protein (OMP) as an adhesion factor and examine its ability to cross-react with the OMPs of other Shigella species.
Methods: OMP was isolated from the bacterium S. flexneri after shaving the pili using a pili bacterial cutter in a solution of 0.5 % n-octyl-a-D-glucopyranoside. Hemagglutination was evaluated using mice erythrocytes. The molecular weight (MW) of the OMPs was profiled by SDS-PAGE. Adhesion molecules were identified by calculating the index of adhesion. Thereafter, antigen cross-reaction with antibodies was examined by Western blotting,
Results: The S. flexneri adhesion molecule OMP has a MW of 28 kDa. An antibody against the 28 kDa S. flexneri OMP adhesion molecule cross-reacted with different MW OMPs from other Shigella species.
Conclusion: This study showed that the 28 kDa S. flexneri OMP is a hemagglutinin adhesion protein, and antibodies against it can cross-react with the OMPs of other Shigella species.

• 出版日期2017-2