A novel fluorescence turn-on probe based on diketopyrrolopyrrole-nitroolefin conjugate for highly selective detection of glutathione over cysteine and homocysteine

作者：Wang, Lingyun^*; Chen, Xianggen; Cao, Derong

来源：Sensors and Actuators B: Chemical , 2017, 244: 531-540.

DOI：10.1016/j.snb.2017.01.024

• Diketopyrrolopyrrole
• Fluorescent probe
• Glutathione
• Michael addition

A new fluorescent probe, nitroolefin functionalized diketopyrrolopyrrole (DPP-NO2) was designed and synthesized as a highly sensitive and selective probe for detecting glutathione (GSH) over cysteine (Cys), homocysteine (Hcy) and other amino acids in aqueous media. The probe is a non-fluorescent compound. On being mixed with GSH in buffered THE/PBS (v/v =3:2) solution at pH 7.2, the probe exhibited the yellow-green emission at 548 nm and a 2000-fold enhancement in fluorescence intensity, along with a 26 nm hypsochromic shift from 515 nm to 489 nm in the absorption spectra. This probe was very sensitive and displayed a linear fluorescence off on response to GSH with fluorometric detection limit of 6.14 x 10(-8) M. What's more, through the fluorescence turn-on signal response to GSH, the probe was successfully utilized for monitoring GSH in living cells.

• 出版日期2017-6
• 单位华南理工大学