科研之友
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摘要
The grape rust mite [Calepitrimerus vitis (Acari: Eriophyidae)] is an important pest of grapevines ( Vitis sp.) in grape-growing regions around the world. A rapid method for extracting eriophyoid mites was adapted from earlier studies to provide integrated pest management (IPM) consultants and commercial growers with a practical, efficient, and reliable tool to monitor grape rust mites in vineyards and nursery stock vines. The rinse in bag (RIB) method allows quick extraction of mites from young shoots or from leaves using 35% to 70% ethanol or isopropanol in a sealable plastic bag. The RIB method recovered approximate to 85% of grape rust mites from single leaves in the first rinse. The method is useful to estimate grape rust mites on young shoots (<= 10 cm length), although recovery of grape rust mites (average ranging from 35% to 81%) was lower because of a higher density of trichomes on young shoots as compared with leaf samples. The MB method was not effective to assess grape rust mites within dormant buds, so a separate method using a blender to disrupt tissues and extract mites in alcohol was developed. The RIB method was used to determine grape rust mite abundance with leaf symptoms in commercial vineyards and nursery stock vines. The earliest visible symptom of grape rust mite damage on leaves in the summer was the development of stippling that is distinct from the type of damage caused by other grapevine pests. The stippling is described as numerous clear zones of small diameter (resembling pinholes) that are visible when a leaf is backlit. The severity of stippling was related to the number of grape rust mites present on leaves, with >600 occurring on leaves with severe stippling symptoms. In commercial vineyard case studies, the RIB method was used over two seasons and revealed that grape rust mite populations remained on leaves until postharvest, and foliar applications of wettable sulfur reduced grape rust mite populations on leaves.
- 出版日期2014-2
