Antioxidant peptides from Cicer arietinum of Xinjiang, China

作者:Yili A*; Ma Q L; Lv Q Y; Gao Y H; Zhao B; Veshkurova O N; Salikhov Sh I; Aisa H A
来源:Chemistry of Natural Compounds, 2012, 48(4): 643-645.
DOI:10.1007/s10600-012-0332-z

摘要

Four peptides of molecular weights 1.148, 4.68, 5.41, and 9.086 kDa with antioxidant activity were isolated from chickpea sprout using two-step ion-exchange chromatographic and HPLC techniques for the first time. The partial N-terminal amino acid sequences of the peptide named YZDBCM-1 (9.086 kDa) was determined as H2N1-15-H2N-Ala-Ile-Thr-Cys-Gly-Arg-Val-Ser-Ala-Ala-Leu-Ala-Pro-Pro-Leu using the Edman automated sequencing apparatus, which was a new peptide rich in alanine. It was shown that the antioxidant activity of the peptide YZDBCM-1 was IC50 156.2 mu g/mL (17.2 mu mol/L) to the free radical of ABTS.

  • 出版日期2012-9
  • 单位中国科学院

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