A rapid method for detection of PrP by surface plasmon resonance (SPR)

作者：Wan Jiayu; Wang Xiong; Li Jiping; Liu Wensen; Xu Ming; Liu Linna; Xu Jing; Wang Haiying; Gao Hongwei^*

来源：Archives of Virology, 2009, 154(12): 1901-1908.

DOI：10.1007/s00705-009-0532-4

Surface plasmon resonance was used to develop a rapid, label-free and sensitive immunoassay for detection of Prion protein (PrP). Anti-PrP monoclonal antibodies immobilized on the biosensor surface were allowed to bind various concentrations of cellular prion protein (PrP(C)), followed by a pulse with additional soluble anti-PrP polyclonal antibodies to intensify the signal. The interaction of antibody with antigen was monitored in real time. With this method, it was possible to detect PrP(C) with a limit of 31.7 ng/ml in serum and 13.1 ng/ml in HEPES-saline, requiring 1 h for a single sample measurement. The assay also detected misfolded prion protein (PrP(Sc)) in spiked serum and PrP(Sc) in scrapie-infected mouse brains. This is a rapid and sensitive assay for the detection of PrP in serum that could be developed into a platform for a serum-based TSE test.

• 出版日期2009-12
• 单位中国人民解放军军事医学科学院