Na-23 double-rotation NMR of sodium nucleotides leads to the discovery of a new dCMP hendecahydrate

作者：Perras Frederic A; Korobkov Ilia; Bryce David L^*

来源：Physical Chemistry Chemical Physics, 2012, 14(14): 4677-4681.

DOI：10.1039/c2cp40273d

Obtaining definitive information concerning the coordination environment of sodium ions which balance the negative charges found in nucleotides is a challenging task. We show that high resolution 1D and 2D Na-23 NMR spectra of sodium nucleotides obtained in the solid state with the use of double-rotation (DOR) provide valuable structural information. Sensitive spin diffusion homonuclear correlation experiments are used to establish the relative proximities of various pairs of crystallographically distinct Na sites and to assign the spectral resonances. Additionally, the DOR sidebands are simulated to obtain coordination information which is complementary to that obtained using multiple-quantum magic-angle spinning NMR spectra. These experiments led us to discover a new hendecahydrate of deoxycytidine monophosphate (dCMP), the structure of which is confirmed via single-crystal X-ray diffraction. This hydrate crystallizes reproducibly when deuterated water is used exclusively in the preparation process.

• 出版日期2012