科研之友
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摘要
Neurogenesis in the dentate gyrus occurs throughout adult mammalian life and is essential for proper hippocampal function. Early in their development, adult-born neurons express homomeric alpha 7-containing nicotinic acetylcholine receptors (alpha 7-nAChRs) and receive direct cholinergic innervation. We show here that functional alpha 7-nAChRs are necessary for normal survival, maturation, and integration of adult-born neurons in the dentate gyrus. Stereotaxic retroviral injection into the dentate gyrus of wild-type and alpha 7-knock-out (alpha 7KO) male and female mice was used to label and birthdate adult-born neurons for morphological and electrophysiological measures; BrdU (5-bromo-2-deoxyuridine) injections were used to quantify cell survival. In alpha 7KO mice, we find that adult-born neurons develop with truncated, less complex dendritic arbors and display GABAergic postsynaptic currents with immature kinetics. The neurons also have a prolonged period of GABAergic depolarization characteristic of an immature state. In this condition, they receive fewer spontaneous synaptic currents and are more prone to die during the critical period when adult-born neurons are normally integrated into behaviorally relevant networks. Even those adult-born neurons that survive the critical period retain long-term dendritic abnormalities in alpha 7KO mice. Interestingly, local infection with retroviral constructs to knockdown alpha 7-mRNA mimics the alpha 7KO phenotype, demonstrating that the relevant alpha 7-nAChR signaling is cell autonomous. The results indicate a profound role for alpha 7-nAChRs in adult neurogenesis and predict that alpha 7-nAChR loss will cause progressive impairment in hippocampal circuitry and function over time as fewer neurons are added to the dentate gyrus and those that are added integrate less well.
- 出版日期2010-6-30
