Assay methods for small ubiquitin-like modifier (SUMO)-SUMO-interacting motif (SIM) interactions in vivo and in vitro using a split-luciferase complementation system

作者：Hirohama Mikako; Voet Arnout R D; Ozawa Takeaki; Saitoh Hisato; Nakao Yoichi; Zhang Kam Y J; Ito Akihiro^*; Yoshida Minoru

来源：Analytical Biochemistry, 2014, 448: 92-94.

DOI：10.1016/j.ab.2013.12.009

• Assay
• SIM
• SUMOylation
• Protein-protein interactions

SUMOylation is a posttranslational process that attaches a small ubiquitin-like modifier (SUMO) to its target proteins covalently. SUMOylation controls multiple cellular processes through the recognition of SUMO by a SUMO-interacting motif (SIM). In this study, we developed assay systems for detecting noncovalent interactions between SUMO and SIM in cells using split-luciferase complementation. We applied a version of this assay to the detection of in vitro SUMO-SIM interactions using a bacterial expression system. These novel assays enable screening of inhibitors of SUMO-dependent protein-protein interactions, either in vivo or in vitro, in a high-throughput manner.

• 出版日期2014-3-1
• 单位RIKEN