A linear plasmid temperature-sensitive for replication in Streptomyces hygroscopicus 10-22

作者：Pang, XH; Sun, YH; Liu, J; Zhou, XF; Deng, ZX^*

来源：FEMS Microbiology Letters, 2002, 208(1): 25-28.

DOI：10.1111/j.1574-6968.2002.tb11055.x

• linear plasmid
• temperature-sensitive
• Streptomyces

Streptomyces hygroscopicus 10-22 harbors a conjugative, autonomously replicating linear plasmid pHZ6 of ca. 70 kb, which shows no obvious homology with chromosomal DNA and is temperature-sensitive for replication, being stable in the host at 28degreesC but easily lost at 37degreesC. On a lawn of the wild-type S. hygroscopicus 10-22 cured of pHZ6, pHZ6 elicit pocks. Temperature sensitivity seemed to be a unique property for pHZ6 among six linear plasmids tested, including the well-known linear plasmids SLP2 in Streptomyces lividans 1326 and SCP1 in Streptomyces coelicolor A3(2). The distinct identity of pHZ6 from previously identified pHZ1-pHZ5 was demonstrated by the profile of relevant plasmids in six well-defined strains originated from S. hygroscopicus 10-22.

• 出版日期2002-2-19
• 单位华中农业大学; 上海交通大学