科研之友
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摘要
The infant formula has been identified as the main transmission vehicle of Cronobacter infections that have been implicated in severe diseases in neonates. Therefore, the accurate detection of Cronobacter is required for microbiological safety of infant formulas. In this study, the duplex-polymerase chain reaction (PCR) targeting a-glucosidase genes was developed and could detect as few as 10(3) cfu/mL in artificially contaminated infant formulas. Positive signals were generated from Cronobacter but not from other non-Cronobacter. Newly developed dot hybridization further confirmed that the PCR was specific to detect Cronobacter in infant formula. Positive fragment sequencing was carried out for phylogenetic analysis and a high heterogeneity between Cronobacter and related species was observed. The phylogenetic analysis is helpful for enriching knowledge of genetic diversity and potential taxonomic complexity.
