A recombinant E-0 gene of bovine viral diarrhea virus protects against challenge with bovine viral diarrhea virus of sika deer

作者：Gao, Yugang; Wang, Yajun; Du, Rui; Wang, Quankai; Zhang, Pengju^*; Wang, Shuzhi; Zhang, Lianxue

来源：African Journal of Microbiology Research, 2011, 5(9): 1012-1017.

• Sika deer
• bovine viral diarrhea virus
• E-0 gene
• eukaryotic expression
• immune response

Eukaryotic expression plasmid (PVAX 1/E-0) of E-0 gene was constructed from an isolated strain of bovine viral diarrhea virus (BVDV) from sika deer and transfected into the BHK-21 cells to detect E-0 gene transcription expression level and protein expression level. It was found that E-0 gene could express in BHK-21 cells by RT-PCR and ELESA results. The humoral and cellular immune response levels were detected in rabbits immunized with PAVX1/E-0 and inactivated vaccine by ELESA technology, which reached the peak after 42 days immunization and PVAX1/E-0 immune rabbits could produce specific higher cells immune responses level in comparison to inactivated vaccine group. This is the first report demonstrating and "immunogenicity" of BVDV E-0 gene on "sika deer", indicating a new vaccine target for BVDV.

• 出版日期2011-5-4
• 单位四川大学