科研之友
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摘要
A fuzzless-lintless (fl) seed mutant of Gossypium hirsutum L. cv. Xu-142 was investigated to study cotton fiber development. Scanning electron microscopy revealed that fiber initials were virtually absent from fl ovules. RT-PCR analysis showed that the steady-state levels of transcripts of the fiber-specific E6 and Expansin genes were high in wild type (WT) ovules during the fiber initiation and elongation stages, and peaked around 15 days post anthesis (DPA), but only a trace amount of these transcripts was detectable in fl ovules of all developmental stages investigated. CotmybA, a member of the Myb family, exhibited a clear expression in developing WT ovules, but the expression was abnormal in fl ovules. Application of GA(3), or GA(3) plus IAA, to the culture medium rescued in vitro fiber initiation and growth of fl ovules only partially In addition, transcription of E6 and Expansin genes of in vitro cultured WT and fl ovules responded similarly to exogenous hormones. The hormones had less effect on CotmybA transcription in ovules cultured in vitro, and again the WT and fl ovules showed a similar expression. These results suggest that the abnormal or extremely low level of expression of E6, Expansin and CotmybA genes in developing fl seeds is related to the absence of seed-hairs, and the mechanism underlying needs further investigation.
- 出版日期2000-12
- 单位南京农业大学; 中国科学院; 中国科学院上海生命科学研究院; 植物分子遗传国家重点实验室
