科研之友
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摘要
The aim of this study was to model the radial growth rate and to assess aflatoxin production by Aspergillus flavus as a function of water activity (aw 0.82 to 0.92) and temperature (12 to 42 degrees C) on polished and brown rice. The growth of the fungi, expressed as colony diameter (mm) was measured daily, and the aflatoxins were analyzed using HPLC with a fluorescence detector. The growth rates were estimated using the primary model of Baranyi, which describes the change in colony radius as a function of time. Total of 2 secondary models were used to describe the combined effects of aw and temperature on the growth rates. The models were validated using independent experimental data. Linear ArrheniusDavey model proved to be the best predictor of A. flavus growth rates on polished and brown rice followed by polynomial model. The estimated optimal growth temperature was around 30 degrees C. A. flavus growth and aflatoxins were not detected at 0.82 aw on polished rice while growth and aflatoxins were detected at this aw between 25 and 35 degrees C on brown rice. The highest amounts of toxins were formed at the highest aw values (0.90 to 0.92) at a temperature of 20 degrees C after 21 d of incubation on both types of rice. Nevertheless, the consistencies of toxin production within a wider range of aw values occurred between 25 to 30 degrees C. Brown rice seems to support A. flavus growth and aflatoxin production more than the polished rice. Practical Application: The developed models can be used to estimate to what extent the change in grain ecosystem conditions affect the storage stability and safety of grains without the need for running long-standing storage study. By monitoring the intergranular relative humidity and temperature at different locations in the storage facility and inputting these data into the models, it is directly possible to assess either the conditions are conductive for the growth of A. flavus or aflatoxin production.
- 出版日期2013-1
