Performance of phosphate-solubilizing bacteria in soil under high phosphorus conditions

作者：De Bolle Sara^*; Gebremikael Mesfin T; Maervoet Veerle; De Neve Stefaan

来源：Biology and Fertility of Soils, 2013, 49(6): 705-714.

DOI：10.1007/s00374-012-0759-1

• PSB
• P saturation
• P mining
• Phosphorus solubilization

A way to bring phosphate-saturated soils back to an environmentally safe P level is by P mining through plants. Phosphate-solubilizing bacteria (PSB) could be very useful for increasing mining efficiency over time. The goal of this research was to investigate the adaptation and performance of PSB in conditions of high total P content in soil. In the first experiment, the P-solubilizing capacity of five PSB species (three Bacillus spp. and two Pseudomonas spp.) were tested under fully controlled conditions on several growth media with different forms of insoluble phosphate (FePO4, AlPO4, or (Ca)(3)(PO4)(2)) added at different rates. The colony growth after 14 days of inoculation demonstrated that all five bacteria were able to proliferate and solubilize P on each of the tested growth media, in contradiction with the normally used technique of halo determination. In the second experiment, the same bacterial species were inoculated in pure quartz sand amended with a nutrient solution and P was added separately in an insoluble form, as Fe-P, Al-P, or Ca-P. The extractable ammonium lactate ranged from 3.2 to 6.9 and 29.0 to 40.7 mg kg(-1) sand for the insoluble Al-P and Fe-P treatments, respectively. Pseudomonas putida and Bacillus brevis performed best as PSB at high P concentration where the P is fixed with Al or Fe. In the third experiment, P. putida and B. brevis were inoculated in an acidic sandy, P-saturated soil for 4 weeks. The inoculation of the PSB gave promising results in solubilizing P.

• 出版日期2013-8