Antibacterial activity of povidone-iodine against an artificial biofilm of Porphyromonas gingivalis and Fusobacterium nucleatum

作者：Hosaka Yasuo; Saito Atsushi; Maeda Ryo; Fukaya Chie; Morikawa Satoru; Makino Asako; Ishihara Kazuyuki; Nakagawa Taneaki^*

来源：Archives of Oral Biology, 2012, 57(4): 364-368.

DOI：10.1016/j.archoralbio.2011.09.005

• Povidone-iodine
• Periodontopathic bacteria
• Biofilm
• Antibacterial agent
• P. gingivalis
• F. nucleatum

Objective: To investigate the antibacterial activity of povidone-iodine (PVP-I) on an artificial dual species biofilm of periodontal pathogens.
Design: Porphyromonas gingivalis or Fusobacterium nucleatum grown in broth culture was inoculated on polycarbonate membrane (PCM) tissue culture inserts. After incubation for 72 h, PVP-I solutions were applied to the biofilm for the time period ranging from 0.5 to 5 min. After addition of a deactivator, each PCM was removed and the biofilm on the PCM was serially diluted and plated on blood agar plates and cultured anaerobically for 7 days. Then viable bacteria were enumerated.
Results: In the dual species biofilm model, F. nucleatum showed an approximately 200-fold increase in viable counts when compared with mono-microbial biofilm. In dual species biofilm, PVP-I with concentration equal to or greater than 2% was required to significantly reduce P. gingivalis and F. nucleatum. When the contact time of PVP-I was increased to 1 min or greater, no difference in antibacterial activity of PVP-I was observed in any concentration.
Conclusion: These results suggest that 30 s application of 2% PVP-I would be effective in suppressing both P. gingivalis and F. nucleatum in dual-species biofilm, and this provides clinical implication for the control of subgingival biofilm.

• 出版日期2012-4