Development of rapid bioconversion with integrated recycle technology for ethanol production from extractive ammonia pretreated corn stover

作者：Jin, Mingjie^*; Liu, Yanping; Sousa, Leonardo da Costa; Dale, Bruce E.; Balan, Venkatesh

来源：Biotechnology and Bioengineering, 2017, 114(8): 1713-1720.

DOI：10.1002/bit.26302

• lignocellulosic ethanol
• RaBIT process
• EA pretreatment
• enzymatic hydrolysis
• enzyme recycling
• ethanol fermentation

High enzyme loading and low productivity are two major issues impeding low cost ethanol production from lignocellulosic biomass. This work applied rapid bioconversion with integrated recycle technology (RaBIT) and extractive ammonia (EA) pretreatment for conversion of corn stover (CS) to ethanol at high solids loading. Enzymes were recycled via recycling unhydrolyzed solids. Enzymatic hydrolysis with recycled enzymes and fermentation with recycled yeast cells were studied. Both enzymatic hydrolysis time and fermentation time were shortened to 24h. Ethanol productivity was enhanced by two times and enzyme loading was reduced by 30%. Glucan and xylan conversions reached as high as 98% with an enzyme loading of as low as 8.4mg protein per g glucan. The overall ethanol yield was 227g ethanol/kg EA-CS (191g ethanol/kg untreated CS). Biotechnol. Bioeng. 2017;114: 1713-1720.

• 出版日期2017-8
• 单位北京化工大学; 南京理工大学