Novel Podoviridae Family Bacteriophage Infecting Weissella cibaria Isolated from Kimchi

作者：Kleppen Hans Petter^*; Holo Helge; Jeon Sang Rok; Nes Ingolf F; Yoon Sung Sik

来源：Applied and Environmental Microbiology, 2012, 78(20): 7299-7308.

DOI：10.1128/AEM.00031-12

The first complete genome sequence of a phage infecting Weissella cibaria (Weissella kimchii) is presented. The bacteriophage phi YS61 was isolated from kimchi, a Korean fermented vegetable dish. Bacteriophages are recognized as a serious problem in industrial fermentations; however, phi YS61 differed from many virulent phages associated with food fermentations since it was difficult to propagate and was very susceptible to resistance development. Sequence analysis revealed that phi YS61 resembles Podoviridae of the subfamily Picovirinae. Within the subfamily Picovirinae, the phi 29-like phages have been extensively studied, and their terminal protein-primed DNA replication is well characterized. Our data strongly suggest that phi YS61 also replicates by a protein-primed mechanism. Weissella phage phi YS61 is, however, markedly different from members of the Picovirinae with respect to genome size and morphology. Picovirinae are characterized by small (approximately 20-kb) genomes which contrasts with the 33,594-bp genome of phi YS61. Based on electron microscopy analysis, phi YS61 was classified as a member of the Podoviridae of morphotype C2, similar to the phi 29-like phages, but its capsid dimensions are significantly larger than those reported for these phages. The novelty of phi YS61 was also emphasized by the low number of open reading frames (ORFs) showing significant similarity to database sequences. We propose that the bacteriophage phi YS61 should represent a new subfamily within the family Podoviridae.

• 出版日期2012-10