Axotomy induces axonogenesis in hippocampal neurons by a mechanism dependent on importin beta

作者：Ohara Ryo; Hata Katsuhiko; Yasuhara Noriko; Mehmood Rashid; Yoneda Yoshihiro; Nakagawa Masanori; Yamashita Toshihide^*

来源：Biochemical and Biophysical Research Communications, 2011, 405(4): 697-702.

DOI：10.1016/j.bbrc.2011.01.108

• Axonal injury
• Axonogenesis
• Dynein
• Importin

We characterize the previously unrecognized phenomenon of axotomy-induced axonogenesis in rat embryonic hippocampal neurons in vitro and elucidate the underlying mechanism. New neurites arose from cell bodies after axotomy and grew. These neurites were Tau-1-positive, and the injured axons showed negative immunoreactivity for Tau-1. Axonogenesis was delayed in these neurons by inhibiting the dynein-dynactin complex through the overexpression of p50. Importin beta, which was locally translated after axotomy, was associated with the dynein-importin a complex and was required for axonogenesis. Taken together, these results suggest that retrograde transport of injury-induced signals in injured axons play key roles in the axotomy-induced axonogenesis of hippocampal neurons.

• 出版日期2011-2-25