Mild hyperthermia enhances the expression and induces oscillations in the Dicer protein

作者：Oshlag Julian Z; Devasthanam Anand S; Tomasi Thomas B^*

来源：International Journal of Hyperthermia, 2013, 29(1): 51-61.

DOI：10.3109/02656736.2012.753471

• Dicer
• microRNAs
• thermal stress
• heat shock response
• oscillations

Purpose: To investigate whether mild heat stress at 39.5 degrees C altered Dicer protein and miRNA expression patterns in several cell types.
Methods: Multiple human and mouse cell types were cultured during the course of 9 h at temperatures from 37 degrees C to 39.5 degrees C. Dicer mRNA levels and microRNAs were quantified by TaqMan RT-qPCR assays and Dicer protein by western blotting.
Results: Dicer protein was substantially elevated on western analysis in response to heat stress at 39.5 degrees C in the absence of significant changes in Dicer mRNA by RT-qPCR.
Conclusions: Heat-induced regulation of Dicer expression occurs primarily post-transcriptionally, and the expression levels of Dicer protein are increased and often oscillate in response to fever-range hyperthermia in multiple mouse and human cells. Our studies suggest a potential role for Dicer and microRNAs in the response to mild thermal stress. Additional studies on the mechanisms involved in the stress-induced oscillations of Dicer protein and microRNAs will be of interest.

• 出版日期2013