科研之友
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摘要
The Clq-domain-containing (ClqDC) proteins, which are involved in various processes of vertebrates, are important pattern recognition receptors in innate immunity of invertebrates. In present study, a novel ClqDC was identified from Mytilus coruscus (designated as McClqDC), which was 917 bp in length encoding 236 amino acids with a typical signal peptide of 19 amino acid residues in N-terminus. Based on its conserved Clq domain and molecular architecture of 10 6-strand jelly-roll folding topology structure, McClqDC might be classified as a member of the Clq family. The mRNA transcript of McClqDC was predominantly detectable in the hemocytes, and a less degree in gill, gonad and mantle, but trace in foot, adductor and digestive gland. Upon induction by Vibrio harveyi and Vibrio alginolyticus, McClqDC expression was significantly up-regulated. Time-dependent mRNA expression of McClqDC was found during copper and cadmium exposure for its heavy metal-binding domain. These results indicated that McClqDC was a novel member of the ClqDC protein family as a pattern recognition receptor against pathogens, and might be developed as a potential indicator for monitoring heavy metals pollution.
